Fluorescence Lifetime Assays

New Technology Enables Primary and Secondary Screening of Broad Panels

Graham Cotton of Almac & Wayne Bowen of TTP Labtech

Published in GEN News 10th May 2013

Luminescence and fluorescence based assay methods prevail in modern HTS. Although invaluable as technologies, the problem of compound-related assay interferences such as quenching, auto-fluorescence, and light scattering becomes important.

The degree of interference experienced may depend on variables such as assay reagents, composition of the chemical library, and reader technology.